Sucrose gradient centrifugation

Sucrose gradient centrifugation is a laboratory technique used to separate molecules, organelles, or particles based on their density, size, or mass. It relies on the creation of a gradient of sucrose concentrations within a centrifuge tube, where samples are subjected to high-speed centrifugation. This method is widely used in molecular biology and biochemistry for purifying macromolecules, subcellular organelles, and viruses.